Immunosuppressant Deoxyspergualin-induced Inhibition of Cell Proliferation is Accompanied with an Enhanced Reduction of Tetrazolium Salt.
نویسندگان
چکیده
منابع مشابه
an investigation of the types of text reduction in subtitling: a case study of the persian film gilaneh with english subtitles
چکیده ندارد.
15 صفحه اولThe immunosuppressant 15-deoxyspergualin [correction of 1,5- deoxyspergualin] reveals commonality between preT and preB cell differentiation
15 [correction of 1,5] deoxyspergualin (DSG) is a potent immunosuppressant whose mechanism of action is still somewhat of a mystery. We have studied the generation of lymphocytes in mice treated with this drug. The differentiation of T cells in the thymus was blocked at an important early control point: the CD4-8- --> CD4+8+ transition, known to depend on the expression of a preTCR complex that...
متن کاملAn improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT.
A new tetrazolium salt XTT, sodium 3'-[1-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6- nitro)benzene-sulfonic acid hydrate, was evaluated for use in a colorimetric assay for cell viability and proliferation by normal activated T cells and several cytokine dependent cell lines. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells yields a highly colored formazan pr...
متن کاملImmunosuppressant deoxyspergualin affects mitochondrial respiratory function in growing cells.
experiments. DSGhas been found to interact intracelluarly with Hsc70, a constitutively expressed memberof the heat shock protein 70 (Hsp 70) family4), but the mechanism of action of DSGstill requires further explanation. We have used methyldeoxyspergualin (MeDSG) for in vitro culture studies of DSG, because it is more stable to hydrolysis than DSG but still possesses a similar immunosuppressive...
متن کاملInhibition of esophageal cancer cell line (KYSE-30) proliferation using secondary metabolites of Ephedra endophyte bacteria
Regarding the rising rate of esophageal cancer in some parts of Asia, including northern and northeastern regions of Iran, the identification of new anti-cancer compounds is essential to combat the disease. The relation between some plants metabolites and these endophytic microorganisms are well characterized. In the current study, the potentials of Ephedra endophyte bacteria for the inhibition...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1999
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.52.45